MANOJ KUMAR (SHELFORD)

Saturday, February 7, 2015

BIOCHEMICAL TEST FOR PRESENCE OF CARBOHYDRATE (QUALITATIVE TEST)

Aim: to test the presence of carbohydrate in given sample

Materials Required: Test tube, test tube holder, sugar solution, Benedict's solution, spirit lamp.

Principle: Sugar contain aldehyde and keto-group which readily reduce metallic oxides, specially cupric to cuprous oxide. In Benedict's  solution CuSO4 is mixed with sodium citrate and sodium carbonate (Na2CO3) which is more stable. In Alkaline Solution all Reducing sugars, glucose, fructose and lactose precipitate Cu2O. In acidic solution only the polysaccharides reduce cupric salt.



observation table
Experiment
Observation
Inference
Clean test tube was taken and 5 ml of Benedict’s reagent was taken in it, 3-4 drops of sample A was added to it, the mixture was thoroughly heated on spirit lamp or boiling water bath
Red or yellow of Cu2O was formed
The given solution contains sugar monosaccharide and reducing disaccharides
Clean test tube was taken and 5 ml of Benedict’s reagent was taken in it, 3-4 drops of sample B was added to it, the mixture was thoroughly heated on spirit lamp or boiling water bath
Yellow ppt not formed
_____


Result: The solution resulted in yellow precipitate formation in sample A, hence carbohydrate is present in sample A









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