Pharmacological Screening of Leaf Extract of Adhatoda vasica for Therapeutic Efficacy

Global Journal of Pharmacology 8(4): 494-500, 2014

ISSN 1992-0075

© IDOSI Publications, 2014

DOI: 10.5829/idosi.gjp.2014.8.4.8419

Pharmacological Screening of Leaf Extract of Adhatoda vasica for Therapeutic Efficacy

ManojKumar, Sukumar Dandapat, Amit Kumar and M.P. Sinha

Department of Zoology, RanchiUniversity, Ranchi-834008, Jharkhand, India

Abstract: In the present study Adhatoda vasica was screened for therapeutic efficacy. The plant leaf extract was screened for phytochemicals, mineral elements, antioxidant and reducing power activity, foaming and swelling index properties. Both qualitative and quantitative analyses of phytochemicals were done. The result showed the presence of alkaloids, tannins, saponins, phenolics and flavonoids. Tannins were maximum among all the detected phytochemicals (61.38 ± 0.8 mg/g). Phenolics were minimum (1.30 ± 0.1 mg/g). Following the presence of phytochemicals the plant leaf samples were also screened for antioxidant and reducing power ability. The screening for mineral elements revealed the presence of macro and micro elements. Potassium (K), Calcium (Ca), iron (Fe), Copper (Cu), Zinc (Zn), Chromium (Cr), Vanadium and Manganese (Mn) were detected in the leaf sample. Adhatoda vasica showed highest (68070 ± 35.58 ppm) concentration of Ca. V was lowest among all the mineral elements (118 ± 6.03 pm). Since the plant contains important phytochemicals, mineral elements, antioxidant and reducing power analysis, it may be good source of future medicines.

 

Key words: Phytochemicals,  Mineral Elements, Antioxidant, Reducing Power

ANTI-HEMOLYTIC ACTIVITY OF ADHATODA VASICA AND VITEX NEGUNDO

Anti-hemolytic activity of Adhatoda vasica and Vitex negundo.

Manoj Kumar, Sukumar Dandapat, Amit Kumar and M. P. Sinha

Department of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India.

Many plants produce biologically active substances, some may be responsible for special flavor or taste and other are/may be useful as antioxidants and/or antimicrobial agents. Antioxidants reduce the damage to cells and biomolecules caused by reactive oxygen species (ROS). The phytochemicals from plants such as phenols, saponins, flavonoids and tannins etc. are well known for their antibacterial and antioxidant properties, some are reported to possess an undesirable hemolytic activity of saponins present in the plant. Thus the present study was taken up to assess the phytochemicals and antioxidant properties of Adhatoda vasica and Vitex negundo and examined the hemolytic activity. The concentration of all the phytochemicals studied was higher in Vitex negundo than Adhatoda vasica, except alkaloids which was higher in Adhatoda vasica (11± 0.1mg/g) than 8.6±0.00 mg/g). Tannins were highest in both the plants, 61.3 ± 0.8mg/g in Adhatoda vasica and 93.9 ± mg/g in Adhatoda vasica. Both plants showed strong antioxidant and reducing power ability. The strong antioxidant and reducing power ability of the plant underlines their use as antioxidant supplement. Both the plants prevented the hemolysis of goat erythrocytes, that is Adhatoda vasica and Vitex negundo exhibited strong anti-hemolytic activity even at higher concentrations, thus they are further certified to be safe and useful for medicinal formulations.

Keywords:  Adhatoda vasica, Vitex negundo, reactive oxygen species, hemolytic activity, antioxidant, reducing

abstract published in Souvenir of International conference on HARMONY WITH NATURE IN CONTEXT OF ECOTECHNOLOGICAL INTERVENTION AND CLIMATE CHANGE. 2013: page  - 72-73

Antibacterial activity of Adhatoda vasica and Vitex negundo

Manoj Kumar

Department of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India

email: scholar.manojkumar@gmail.com 

The methanolic leaf extract of Vitex negundo and Adhatoda vasica were subjected to phytochemical, In-vitro antioxidant power analysis. The phytochemical studies showed the presence of alkaloids, tannins, flavonoids and saponins. The antioxidant activity was determined and compared with Butyl Hydroxy Anisole (BHA) as standard. The reducing power ability of both the plants were determined and compared with ascorbic acid as standard. Quantitative studies were also done. The extract was tested for antibacterial properties against five bacteria viz. Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Proteus mirabilis, Salmonella typhi Agar disc diffusion method and broth dilution method. The inhibition of microorganisms were compared within both plants and with pure antibiotic Gentamycin. The results exhibited strong antibacterial activity of Adhatoda vasica and Vitex negundo against B. subtilis and P. mirabilis. The minimum inhibitory concentration ranged from 0.612mg/ml in case of Vitex negundo and 2.5mg/ml in case of Adhatoda vasica.

Key words: Minimum inhibitory concentration, BHA, Gentamycin, antibacterial, antioxidant, phytochemicals

abstract published in Souvenir of International conference on HARMONY WITH NATURE IN CONTEXT OF ECOTECHNOLOGICAL INTERVENTION AND CLIMATE CHANGE. 2013: page  - 72 

PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENCY OF ADHATODA VASICA AND VITEX NEGUNDO

8(2): 727-730, 2013, The Bioscan (Supplement on Medicinal Plants)

PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENCY OF ADHATODA VASICA AND VITEX NEGUNDO

Manoj Kumar*, Amit Kumar, Sukumar Dandapat, M. P. Sinha

*Corresponding author email: eaddress.manojkumar@gmail.com

ABSTRACT

The leaf samples of Adhatoda vasica and Vitex negundo were subjected to phytochemical analysis. Both the plants contained antioxidant phytochemicals such as alkaloids, tannin, saponins, phenolics and flavonoids; which were present in comparatively higher amount in Vitex negundo. Tannins were recorded highest among all the phytochemicals (93.9 ± 0.8 and 61.3 ± 0.8 mg/g in Vitex negundo and Adhatoda vasica respectively). Phenolics were recorded lowest (13. ± 0.1 and 8.1 ± 0.5 mg/g in Adhatoda vasica and Vitex negundo respectively.The methanolic extracts of the plants were also analyzed for antioxidant and reducing power potentiality. Both plants showed strong antioxidant and reducing power ability. The strong antioxidant and reducing power ability of the plant underlines their use as antioxidant supplement against diseases such as typhoid during which antioxidant system fails; cardiovascular diseases which are caused due to accumulation of Reactive oxygen species; ageing related diseases, Alzheimer, Prkinson’s disease, Amytrophic lateral sclerosis, cataractogenesis and other diseases.

KEY WORDS: antioxidant, Adhatoda vaisca, Vitex negundo

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Anti-typhoid Activity of Adhatoda vasica and Vitex negundo

Persian Gulf Crop Protection

Available online on: www.cropprotection.ir

ISSN: 2251-9343 (Online)

Volume 2 Issue 3, September 2013

Pages 64-75

Anti-typhoid Activity of Adhatoda vasica and Vitex negundo

Manoj Kumar1*, Sukumar Dandapat1, Amit Kumar1 and M. P. Sinha1

1-Department of Zoology, Ranchi University, Ranchi, India (*Corresponding author e-mail:eaddress.manojkumar@gmail.com).

Abstract: Typhoid activity is an acute systemic infection caused by Salmonella typhi. In present study

the methanolic leafs extract of Vitex negundo and Adhatoda vasica were analyzed for anti-typhoid

activity against Salmonella typhi. The leave sample was subjected to phytochemical analysis. The

concentration of all the phytochemicals studied was higher in Vitex negundo than Adhatoda vasica,

except alkaloids which was higher in Adhatoda vasica (11.3 ± 0.1mg/g) than Vitex negundo (8.6 ± 0.00

mg/g). Tannins were highest in both the plants, 61.3 ± 0.8 mg/g in Adhatoda vasica and 93.9 ± 0.8

mg/g in Adhatoda vasica. The antioxidant activity was determined and compared with BHA and

reducing power was compared with Ascorbic acid. Both the samples had high antioxidant and reducing

power activity. Several studies reported the loss of antioxidant system during infection of Salmonella

typhi. The leaf extracts of Adhatoda vasica and Vitex negundo showed considerable antioxidant activity

which can used as remedy against antioxidant system collapse and thus promises to be effective

antioxidant supplement for typhoid patients. Besides antioxidant activity the leaves of both plants

inhibited the growth of Salmonella typhi. The antibacterial activity of both leaf extracts were compared

with gentamycin. The results of present study shows that leaf extracts of Vitex negundo and Adhatoda vasica

confer anti-typhoid activity against Salmonella typhi.

Key Words: Anti-typhoid, Adhatoda vasica, Vitex negundo.

Determination of Nutritive Value and Mineral Elements of Five-Leaf Chaste Tree (Vitex negundo L.) And Malabar Nut (Adhatoda vasica Nees)

Academic Journal of Plant Sciences 6 (3): 103-108, 2013
ISSN 1995-8986
© IDOSI Publications, 2013
DOI: 10.5829/idosi.ajps.2013.6.3.11011

Determination of Nutritive Value and Mineral Elements of

Five-Leaf Chaste Tree (Vitex negundo L.) And Malabar Nut

(Adhatoda vasica Nees)

Manoj Kumar*, Sukumar Dandapat, Amit Kumar and M.P. Sinha

Department of Zoology, Ranchi University, Ranchi - 834008, India.

Abstract: 

This study was undertaken to assess the nutritive value and mineral contents from Vitex negundo and Adhatoda vasica. These two plant species are fairly used as medicine throughout the greater part of India. Adhatoda vasica is used to control pain, inflammation and other related diseases. Leaves of Adhatoda vasica are used for treatment of cold, cough, chronic bronchitis and asthma. It was also used by traditional midwives at the time of delivery. The leaves of Adhatoda vasica are extensively used in indigenous medicines remedies. Both the plants contained important macro and micro elements: K, Ca, Fe, Cu, Zn and Cr. These elements were found in more quantity in Vitex negundo than in Adhatoda vasica. The leaves of both the plants were analyzed for ash content, moisture, crude fat, crude fibre, crude carbohydrate and crude protein content. The results for percentage of ash content, moisture content, crude fat, crude fiber, carbohydrate and protein were 5.4 ± 0.35, 16.50 ± 1.2, 7 ± 0.7, 28.02 +1.03, 8.5 ± 0.45, 13.7 ± 1.04 % respectively for Vitex negundo; and 5.2 ± 1.23, 15.3 ± 0.5, 1.6 ± 0.3, 6.4 ± 0.45, 16.4 ± 0.8, 6.5 ± 0.3 respectively for Adhatoda vasica. The leaves were also assessed for nutritional value. Nutritional value of Vitex negundo was 151.80 Cal/100g and that of Adhatoda vasica was 106.00 Cal/100.g.

Key words:

Vitex negundo, Adhatoda vasica, Nutritive value, Mineral contents

Corresponding Author: Manoj Kumar, Department of Zoology, Ranchi University, Ranchi - 834008, India.

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