MANOJ KUMAR (SHELFORD)

Monday, September 9, 2013

Anti-typhoid Activity of Adhatoda vasica and Vitex negundo

Persian Gulf Crop Protection
Available online on: www.cropprotection.ir
ISSN: 2251-9343 (Online)
Volume 2 Issue 3, September 2013
Pages 64-75

Anti-typhoid Activity of Adhatoda vasica and Vitex negundo

Manoj Kumar1*, Sukumar Dandapat1, Amit Kumar1 and M. P. Sinha1

1-Department of Zoology, Ranchi University, Ranchi, India (*Corresponding author e-mail:eaddress.manojkumar@gmail.com).

Abstract: Typhoid activity is an acute systemic infection caused by Salmonella typhi. In present study
the methanolic leafs extract of Vitex negundo and Adhatoda vasica were analyzed for anti-typhoid
activity against Salmonella typhi. The leave sample was subjected to phytochemical analysis. The
concentration of all the phytochemicals studied was higher in Vitex negundo than Adhatoda vasica,
except alkaloids which was higher in Adhatoda vasica (11.3 ± 0.1mg/g) than Vitex negundo (8.6 ± 0.00
mg/g). Tannins were highest in both the plants, 61.3 ± 0.8 mg/g in Adhatoda vasica and 93.9 ± 0.8
mg/g in Adhatoda vasica. The antioxidant activity was determined and compared with BHA and
reducing power was compared with Ascorbic acid. Both the samples had high antioxidant and reducing
power activity. Several studies reported the loss of antioxidant system during infection of Salmonella
typhi. The leaf extracts of Adhatoda vasica and Vitex negundo showed considerable antioxidant activity
which can used as remedy against antioxidant system collapse and thus promises to be effective
antioxidant supplement for typhoid patients. Besides antioxidant activity the leaves of both plants
inhibited the growth of Salmonella typhi. The antibacterial activity of both leaf extracts were compared
with gentamycin. The results of present study shows that leaf extracts of Vitex negundo and Adhatoda vasica
confer anti-typhoid activity against Salmonella typhi.

Key Words: Anti-typhoid, Adhatoda vasica, Vitex negundo.

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