MANOJ KUMAR (SHELFORD)

Showing posts with label ANTI-HEMOLYTIC. Show all posts
Showing posts with label ANTI-HEMOLYTIC. Show all posts

Sunday, November 17, 2013

ANTI-HEMOLYTIC ACTIVITY OF ADHATODA VASICA AND VITEX NEGUNDO

Anti-hemolytic activity of Adhatoda vasica and Vitex negundo.
Manoj Kumar, Sukumar Dandapat, Amit Kumar and M. P. Sinha
Department of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India.

Many plants produce biologically active substances, some may be responsible for special flavor or taste and other are/may be useful as antioxidants and/or antimicrobial agents. Antioxidants reduce the damage to cells and biomolecules caused by reactive oxygen species (ROS). The phytochemicals from plants such as phenols, saponins, flavonoids and tannins etc. are well known for their antibacterial and antioxidant properties, some are reported to possess an undesirable hemolytic activity of saponins present in the plant. Thus the present study was taken up to assess the phytochemicals and antioxidant properties of Adhatoda vasica and Vitex negundo and examined the hemolytic activity. The concentration of all the phytochemicals studied was higher in Vitex negundo than Adhatoda vasica, except alkaloids which was higher in Adhatoda vasica (11± 0.1mg/g) than 8.6±0.00 mg/g). Tannins were highest in both the plants, 61.3 ± 0.8mg/g in Adhatoda vasica and 93.9 ± mg/g in Adhatoda vasica. Both plants showed strong antioxidant and reducing power ability. The strong antioxidant and reducing power ability of the plant underlines their use as antioxidant supplement. Both the plants prevented the hemolysis of goat erythrocytes, that is Adhatoda vasica and Vitex negundo exhibited strong anti-hemolytic activity even at higher concentrations, thus they are further certified to be safe and useful for medicinal formulations.

Keywords:  Adhatoda vasica, Vitex negundo, reactive oxygen species, hemolytic activity, antioxidant, reducing

abstract published in Souvenir of International conference on HARMONY WITH NATURE IN CONTEXT OF ECOTECHNOLOGICAL INTERVENTION AND CLIMATE CHANGE. 2013: page  - 72-73

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