Anti-hemolytic
activity of Adhatoda vasica and Vitex negundo.
Manoj
Kumar, Sukumar Dandapat, Amit Kumar and M. P. Sinha
Department
of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India.
Many plants produce
biologically active substances, some may be responsible for special flavor or
taste and other are/may be useful as antioxidants and/or antimicrobial agents. Antioxidants
reduce the damage to cells and biomolecules caused by reactive oxygen species
(ROS). The phytochemicals from plants such as phenols, saponins, flavonoids and
tannins etc. are well known for their antibacterial and antioxidant properties,
some are reported to possess an undesirable hemolytic activity of saponins
present in the plant. Thus the present study was taken up to assess the
phytochemicals and antioxidant properties of Adhatoda vasica and Vitex negundo
and examined the hemolytic activity. The concentration of all the
phytochemicals studied was higher in Vitex negundo than Adhatoda vasica, except
alkaloids which was higher in Adhatoda vasica (11± 0.1mg/g) than 8.6±0.00
mg/g). Tannins were highest in both the plants, 61.3 ± 0.8mg/g in Adhatoda
vasica and 93.9 ± mg/g in Adhatoda vasica. Both plants showed strong antioxidant
and reducing power ability. The strong antioxidant and reducing power ability
of the plant underlines their use as antioxidant supplement. Both the plants
prevented the hemolysis of goat erythrocytes, that is Adhatoda vasica and Vitex
negundo exhibited strong anti-hemolytic activity even at higher concentrations,
thus they are further certified to be safe and useful for medicinal
formulations.
Keywords: Adhatoda vasica, Vitex negundo, reactive
oxygen species, hemolytic activity, antioxidant, reducing
abstract published in Souvenir of International conference on HARMONY WITH NATURE IN CONTEXT OF ECOTECHNOLOGICAL INTERVENTION AND CLIMATE CHANGE. 2013: page - 72-73
