MANOJ KUMAR (SHELFORD)

Wednesday, August 17, 2011


Aim: estimation of protein.
Method: blank standard test.
Principle:
Protein + Cu2+        alkaline medium         Cu-Protein complex
Requirements: three test tubes, micropipette-20 micro liter and 100 micro liter, calorimeter, beaker, micro pipette tips,
Reagents:
Burette reagent, protein, standard, serum, distilled water.
The reagents contain:
1.        Copper sulphate solution (12 mmol)
2.        Potassium iodide ( 20 mmol)
3.        NaOH (600 mmol)
4.        Sodium potassium tartarate (32 mmol)
Procedure:
a.        Calorimeter was switched ON and left ON for 1 hour before the experiment.
b.        Three test tubes were taken and marked as B (blank), T(test tube), S (standard).
c.        Calibration of calorimeter: calorimeter was set to zero i.e. 100% transmittance by the blank solution. Blank solution is prepared by taking 1000 micro liter burette reagent and 20 micro liter of distilled water in test tube.
d.        Now 1000 micro liter of burette reagent was taken in test tube S and T.
e.        Then 20 micro liter of protein standard was added to s marked test tube and 20 micro liter of serum was added in T marked test tube by the help of micropipette.
f.         After mixing the contents of test tube properly, the test tubes were incubated at room temperature for five minutes.
g.        Now the absorbance of T and S test tube solution was measured.

B
S
T
BIURETE READING
1000 µl
1000 µl
1000 µl
PROTEIN STANDARD
------
20 µl
------
SERUM
------
------
20 µl
DISTILLED WATER
20 µl
------
------

Observation:
Serial number
Absorbance of S
Absorbance of T
1
0.26
0.22
2
0.29
0.27
3
0.12
0.09
4
0.21
0.19
5
0.23
0.21

Calculations:
                                                =
                                                =
                                                =4.4 mg/dl

No comments:

Post a Comment

Blog Archive

Labels

2012 4TH LARVAL AND PUPAL STAGES. manoj kumar adaptations ADHATODA VASICA agarose agarose gel electrophoresis. agaros gel electrophoresis theory allergic reactions allergy amino acids anopheles ANSWER KEYS CSIR antheraea mylitta ANTI-HEMOLYTIC anti-typhoid antibacterial antibody anurans aquatic aquatic mammals arthropoda autotrophs BACTERIAL DISEASES BAT BEHAVIOUR benedict reagent benedict's reagent benedict's test benedicts reagent biochemistry bioscan biotechnology BIRDS bis bizzare phenomena blood blood sucking blood sucking bugs bruce effect bugs carbohydrate carbon dioxide CARNIVORUS. CAT CBSE CBSE 2014 CBSE EXAM CBSE SEMESTER II SCIENCE cbsex census 2011 centepede central board of secondary education chapter chapter 2 chapter2 class class 6 CLASS 7 class x class6 CLASS7 classical genetics CLASSIFICATION classification of amino acids cloning vectors COMMON DISEASES conference india CSIR WEB LINKS cuscuta dav dempster department of zoology Digestive System DNA RECOMBINANT TECHNOLOGY DOG domestic domestic levels. india DOWNLOAD NET ANSWER KEYS DOWNLOAD NET QUESTION PAPERS drury haemocytes drury hemocytes ecology ecoscan electrophoresis elements of innate immune system energy entomology environmental biology. exam extraction farmers fat father female anopheles fertilisers fine structure of antibody FMD food food chain FOOT AND MOUTH DISEASE Franz frog vocal FUNCTIONS fut content analysis GE02 gel genetic engineering genetics giardia giardiasis glucose test gorakhpur conference 2013 gram stain harmony - 2013 harmony 2-13 HARMONY 2014 harmony-2013 harmony2013 hemiptera heteroptera heterotrophs house centipede hypersensitivity immune system immunology in in plants India inheritance innate inportance INSECT ENDOCRINOLOGY isotypes Jhakrhand kwashiorkor lamblia larval stage law of minimum leibig levels limiting factors M. P. SINHA M. P. SINHA. malaria malarial malnutrition mammals mammals. manoj kumar manoj kumar shelford manoj kumar zoology manoj kumar zoology ranchi marasmus MATING microbiology MINERAL CONTENTS minerals MODE OF ACTION OF RABIES VIRUSE molecular genetics Mp sinha national environmentalists association ncert nea NEA CONFERENCE nea conference 2013 NEST nutrition nutrition in NUTRITIVE VALUE origin and evolution of reptiles oxygen parasite PARENTAL CARE IN BIRDS pcr pharmacological photosynthesis physical barriers PHYTOCHEMICALS PLACENTA PLACENTA VERA plants plasmids plasmodium polyadenylation. polymerase chain reaction potable potable water precipitaion test pregnancy block proper growth protein protein energy malnutrition proteins PROTOZOAN DISEASE purification qpcr qualitative test RABIES RANCHI UNIVERSITY RANCHI. revesion test RHEOCIRUSE SA -b 1SCIENCE SA CBSE SCIENCE SA-II SCIENCE SAMPLE PAPER CLASS X saprotrophs science SCIENCE CBSE science class x Scoliodon Scoliodong ppt scutigera sex linked inheritance shelford significance of foood chain soxhlet soxhlet extraction soxhlet. soxlet extraction stanley cohen STUDIES ON ANTHERAEA MYLITTA DRURY HEMOCYTES DURING 3RD sucking sucking bugs Sukumar Dandapat summative assesment summative assesment - II summative assesment 2012 synce ten trap cropping TYPES TYPHOID UDAIPUR UMBILICAL CORD use of radioactive isotopes vectors VIRUS. VIRUSE vitamins VITEX NEGUNDO vocalisation in amphibia vocalization in amphibians Von water web