Antibacterial activity of Adhatoda vasica and Vitex negundo

Manoj Kumar

Department of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India

email: scholar.manojkumar@gmail.com 

The methanolic leaf extract of Vitex negundo and Adhatoda vasica were subjected to phytochemical, In-vitro antioxidant power analysis. The phytochemical studies showed the presence of alkaloids, tannins, flavonoids and saponins. The antioxidant activity was determined and compared with Butyl Hydroxy Anisole (BHA) as standard. The reducing power ability of both the plants were determined and compared with ascorbic acid as standard. Quantitative studies were also done. The extract was tested for antibacterial properties against five bacteria viz. Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Proteus mirabilis, Salmonella typhi Agar disc diffusion method and broth dilution method. The inhibition of microorganisms were compared within both plants and with pure antibiotic Gentamycin. The results exhibited strong antibacterial activity of Adhatoda vasica and Vitex negundo against B. subtilis and P. mirabilis. The minimum inhibitory concentration ranged from 0.612mg/ml in case of Vitex negundo and 2.5mg/ml in case of Adhatoda vasica.

Key words: Minimum inhibitory concentration, BHA, Gentamycin, antibacterial, antioxidant, phytochemicals

abstract published in Souvenir of International conference on HARMONY WITH NATURE IN CONTEXT OF ECOTECHNOLOGICAL INTERVENTION AND CLIMATE CHANGE. 2013: page  - 72