MANOJ KUMAR (SHELFORD)

Wednesday, June 4, 2014

The Ecoscan: volume IV (special issue); 241-246

GROWTH INHIBITORY IMPACT OF ADHATODA VASICA AND VITEX NEGUNDO ON SOME HUMAN PATHOGENS

MANOJ KUMAR*, SUKUMAR DANDAPAT, AMIT KUMAR, M. P. SINHA
Zoology Department of Ranchi University, Ranchi
*e-mail: eaddress.manojkumar@gmail.com.

ABSTRACT
The present study was undertaken to determine the growth inhibitory impact of Adhatoda vasica and Vitex negundo on five human pathogens Viz. Salmonella typhi, Pseudomonas, aeruginosa, Proteus mirabilis, Bacillus subtilis, Staphylococcus aureus which are known to cause several diseases and infections in human beings such as typhoid fever, urinary tract infection, pulmonary tract infection, specticemia, pneumonia, staphylococcal scalded skin syndrome (SSSS) by agar disc diffusion method and broth dilution method. The minimum inhibitory concentration of Vitex negundo ranged from 0.612 mg/mL (Bacillus subtilis) to 5 mg/mL (Pseudomonas aeruginosa and Salmonella typhi). The minimum inhibitory concentration of Adhatoda Vasica ranged from 2.5 mg/mL (Bacillus subtilis) to 5 mg/mL (Staphylococcus aureus and Pseudomonas aeruginosa). The plants were also screened for their phytochemical contents and presence of alkaloids, tannin, saponin and flavonoids were determined. Both the plants showed growth inhibitory impact on the tested bacteria and possess potent antibacterial phytochemicals, thus Adhatoda vasica and Vitex negundo can help to discover new chemical classes.

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